Figure 3 | Scientific Reports

Figure 3

From: Prokineticin receptor-1-dependent paracrine and autocrine pathways control cardiac tcf21+ fibroblast progenitor cell transformation into adipocytes and vascular cells

Figure 3

Regulation of the transformation of CF-like cells into adipocytes by a cardiomyocyte-mediated paracrine pathway. (A) Schematic illustration of the experimental design. Adult CF-like cells were cultured in the conditioned medium of PKR1-overexpressing cardiomyocytes (infected with adv-PKR1) (CM++-M) to mimic TG mouse models. Conditioned medium of cardiomyocytes (CM-M) infected with Adv-control was used as a control. (B) Oil Red O staining of CF-like cells cultured in CM-M alone or in CM-M supplemented with an adipogenic induction cocktail (AIC) (CM-M+ AIC), CM++-M alone or supplemented with AIC (CM++-M+ AIC) in the presence or absence of anti-PK2 antibody (α-PK2). (C) Endothelial-specific Flk-1 or smooth muscle actin (α-SMA) staining of CF-like cells following the treatments described above. Adipocyte (D), SMC (E) and endothelial cell (F) numbers are presented as histograms (*p < 0.05, compared with vehicle-treated cells; **p < 0.05, compared to AIC-treated cells; n = 6, 10 pictures per condition, unpaired two-tailed Student’ s t-Test). All n = 12 mice/group. The drawing in 3A was created using Servier Medical Art illustration resources (www.servier.com).

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