Table 2 Primers used for real-time PCR and genotyping.
Gene name | Forward | Reverse |
|---|---|---|
Tcf21iCre | GCTTCCGATATCCAGATCCAGAC | CAAACCCTAGCACAAATCACTCGC |
PKR1fl/fl | GACTGGACATCTAGTGGTAGTCAGG | GGGTGTGAGGTGGGATTAAGTCAC |
β actin | CATCTTGGCCTCACTGTCCA | GGGCCGGACTCATCGTACT |
GAPDH | AACGACCCCTTCATTGAC | TCCACGACATACTCAGCAC |
C/EBPβ | CAAGCTGAGCGACGAGTACA | CAGCTGCTCCACCTTCTTCT |
Fabp4/aP2 | ACA CCG AGA TTT CCT TCA AAC TG | CCA TCT AGG GTT ATG ATG CTC TTC A |
Scd-1 | TCTGGGAGAGTGCTGACAAAAA | CTGCTGAGGATCCCCAAATACT |
Adiponectin | GCACTGGCAAGTTCTACTGCAA | GTAGGTGAAGAGAACGGCCTTGT |
PPARγ | GTGCCAGTTTCGATCCGTAGA | GGCCAGCATCGTGTAGATGA |
Perilipin | GGCCTGGACGACAAAACC | CAGGATGGGCTCCATGAC |
Ucp-1 | ACT GCC ACA CCT CCA GTC ATT | CTT TGC CTC ACT CAG GAT TGG |
Tie-2 | ATGTGGAAGTCGAGAGGCGAT | CGAATAGCCATCCACTATTGTCC |
Flk-1 | GCCCTGCTGTGGTCTCACTAC | CAAAGCATTGCCCATTCGAT |
Pecam-1 | GAGCCCAATCACGTTTCAGTTT | TCCTTCCTGCTTCTTGCTAGCT |
SM-MHC | GGTCGTGGAGTTGGTGGAAA | CTGCCATGTCCTTCCACCTTAG |
Calponin | AGGCCAACGACCTGTTTGAA | CACATTGACTTTGTTTCCTTTTGTCT |
Fibronectin | ATGTGGACCCCTCCTGATAGT | GCCCAGTGATTTCAGCAAAGG |
Col1a1 | GCCAAGAAGACATCCCTGAAG | TGTGGCAGATACAGATCAAGC |
