Figure 7 | Scientific Reports

Figure 7

From: CCAAT/Enhancer binding protein β silencing mitigates glial activation and neurodegeneration in a rat model of Parkinson’s disease

Figure 7

Injection of lentiviral particles containing C/EBPβ shRNA in the Substantia nigra pars compacta of adult rats significantly reduces pro-inflammatory factors production in a in vivo model of Parkinson’s disease. Rats were injected into the SNpc with lentiviral particles containing Non-Targeting shRNA (NT-shRNA) or C/EBPβ-shRNA in combination or not with 6-OHDA (5 μg). After 7 (a,c) and 15 (b,d) days animals were sacrificed, and the brains processed for immunofluorescence, as indicated in Methods. Double immunofluorescence representative images of both hemispheres (contra- and ipsilateral) showing pro-inflammatory interleukin 1β (IL1β) (a,b) or ciclooxigenase 2 (COX-2) (c,d) expression (green) together with the dopaminergic marker tyrosine hydroxylase (TH, red). Nuclei were stained with DAPI. Insets show high magnification of the selected area. Scale bar = 100 μm. Scale bar (insets in a and b) = 50 μm.

Back to article page