Figure 3 | Scientific Reports

Figure 3

From: Mir-21 Mediates the Inhibitory Effect of Ang (1–7) on AngII-induced NLRP3 Inflammasome Activation by Targeting Spry1 in lung fibroblasts

Figure 3

ACE2/Ang(1–7) inhibited AngII-induced apoptosis resistance and collagen synthesis by inhibiting the mir-21-mediated Spry1/ERK/NF-κB NLRP3 inflammasome pathway. Primary lung fibroblasts were infected with lentiACE2 or pretreated with Ang(1–7) and then exposed to AngII. (A) The mir-21 levels were detected by qRT-PCR. (B) The protein levels of Spry1 p-ERK and NF-κB were analyzed by western blot. (C) The protein levels of NLRP3, pro-casp-1, cleaved casp-1, pro-IL-1β, and active IL-1β were analyzed by western blot. (D) Apoptosis of cells was evaluated by flow cytometry. Data are the means ± SD from 3 independent experiments. *P < 0.05 versus control; # P < 0.05 versus AngII; † P < 0.05 versus lentiNC; ‡ P < 0.05 versus AngII + lentiNC.

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