Figure 4 | Scientific Reports

Figure 4

From: Mir-21 Mediates the Inhibitory Effect of Ang (1–7) on AngII-induced NLRP3 Inflammasome Activation by Targeting Spry1 in lung fibroblasts

Figure 4

ACE2/Ang-(1–7) had no influence on the downstream pathway of mir-21. Primary lung fibroblasts were transfected with mir-21 precusors (pre-21) after the cells had been transfected with lentiACE2 or pretreated with Ang(1–7) for 24 h. (A) The mir-21 levels were detected by qRT-PCR. (B–D) The protein levels of Spry1, p-ERK, NF-κB, NLRP3, Pro-casp-1, cleaved casp-1, Pro-IL-1β, active IL-1β and a-collagen I were analyzed by western blot. Data are the means ± SD from 3 independent experiments. *P < 0.05 versus pre-NC.

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