Figure 5 | Scientific Reports

Figure 5

From: Unusual isothermal multimerization and amplification by the strand-displacing DNA polymerases with reverse transcription activities

Figure 5

Sensitivity, specificity, universality assay of UIMA. All reactions were performed at 63 °C for 180 min. (A) Sensitivity of UIMA using 1.6 μM RL primer and different concentration (from 10 nM to 10pM) of F*R* template with 10-fold dilution. (B) Specificity of UIMA with 1.6 μM RL primer to assay different templates including 10 nM F*R*, 10 nM synthesized ssDNA partially obtained from genomic DNA of HPV 18 subtype (HPV 18), 1 ng Lamder DNA, 2 ng human genomic DNA (gDNA), and the mixtures of 10 nm F*R* and 2 ng gDNA. (C) and (D) Universality of UIMA with 1.6 μM corresponding primers to detect 10 nM templates of HBV DNA, HPV 18 DNA, and their mixtures with gDNA. HBV DNA was a synthesized ssDNA from a part of genomic HBV DNA.; NTC: no-template control; NPC: no-primer control. Each reaction was in triplicate. Detailed sequences were shown in Table S1.

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