Figure 1
From: Unveiling some FDA-approved drugs as inhibitors of the store-operated Ca2+ entry pathway
Evaluation of the effects of chosen drugs on the store-operated calcium entry (SOCE) in RBL-1 cells triggered by thapsigargin using Fura-2 based Ca2+ imaging experiments. (a) Sample traces representing Ca2+ signals (indicated by Fura-2 fluorescence ratio) triggered by adding 2 μM of thapsigargin (Tg) to RBL-1 cells with or without pre-treatment of the chosen drugs (shown in various coloured lines, each at 100 μM dose). (b) Histograms showing the peak SOCE levels triggered by Tg in control cells and cells treated with 100 μM of each drug. (c) Histograms showing the peak SOCE levels triggered by Tg in control cells and cells pre-treated with each drug at 10 μM dose. Each value (mean ± SEM) was derived from 3–5 individual experiments and a total of 30–80 cells. The statistical comparison among the values for all conditions was determined using one way ANOVA followed by Dunnett’s test.
