Figure 7
Identification and characterization of CTIP1 target genes in murine embryonic skin (a) Distribution of CTIP1 ChIP-seq peaks over proximal promoters (≤2 kb upstream of TSS), distal promoters (2–50kb), exons, introns, and intergenic regions. (b) Venn diagram represents the proportion of differentially expressed genes in Ctip1 −/− embryonic skin (RNA-seq) with enrichment for CTIP1 peak by ChIP-seq. (c,f) ChIP-qPCR validation of ChIP-seq peaks at the ~1.5 kb upstream of TSS of Fosl2 (c) and ~3 kb upstream of TSS of Elovl4 (f). (d,g) Immunoblot of ventral skin extracts from Ctip1 +/+ and Ctip1 −/− embryos at E18.5 using antibodies against FOSL2 (d) and ELOVL4 (g). β-actin was used as a loading control. Full-length blots are presented in Supplementary Figure S7. (e,h) Quantification of Fosl2 (e) and ELOVL4 (h) immunoblots, *P < 0.05, **P < 0.01.
