Figure 5
From: Classical swine fever virus nonstructural protein p7 modulates infectious virus production
The interaction between E2p7 and p7NS2. (A) The interaction between E2p7 and p7NS2 by a Co-IP assay. 293 T cells transfected with indicated plasmids were lysed and immunoprecipitated with an anti-Myc antibody. (B) The subcellular co-localization between E2p7 and NS2 in PK15 cells. PK15 cells transfected with indicated plasmids were immunostained with both an anti-Myc antibody (green) and an anti-HA antibody (red) at 24 hpt. The nuclei were stained with DAPI. Scale bar, 10 μm. (C) The degree of co-localization was quantified by determining Pearson’s correlation coefficients. For each sample 30 cells were quantified using Image Pro Plus software. Data are represented as the mean values (±SD) from three independent experiments. *P < 0.05; **P < 0.01; ns, not significant.
