Figure 3
PLIMB identifies regions previously implicated in EGF-induced structural changes. (A) Close up view of residues within cluster at top of dimerization interface, shown in the crystal structure. These were identified as decreased in oxidation state when EGF is added to EGFR in solution in the experiments described herein. Green and yellow are the residues on either monomer of the dimer, and residue numbers are labeled. (B) Close up view of “hinge region” spanning Domains II and III, for which arrangement changes upon binding EGF. Domain I is shown in red, Domain II green, and Domain III blue. Domain IV is not pictured. Residues for which oxidation decreased upon adding EGF are shown in yellow and labeled. Image of 1IVO15 created using PyMOL (The PyMOL Molecular Graphics system, Version 1.8, Schrödinger, LLC).
