Figure 5

(A) X-band continuous wave (cw) EPR spectra of parental iLOV (black trace) and iLOV-Q489D (blue trace) recorded after 20 min illumination of the dark adapted samples. The green trace shows the EPR spectrum of illuminated iLOV-Q489D after annealing for 10 min at 240 K. All spectra were acquired at 120 K. (B) Electron spin echo nutation time traces and (C) their Fourier transforms for the illuminated iLOV-Q489D sample recorded at the magnetic field positions marked by arrows in the inset panel (C) which shows field-swept echo-detected EPR spectrum at 120 K. The dashed lines in (C) mark the frequency positions of FFT amplitude maxima. The frequency axis is normalized to the frequency of the low frequency peak in the upper trace.