Figure 8
Superposition of representative LOV domain structures (A) and sequence logo generated to visualize the sequence conservation within the LOV domain family (B). The structure of the parent iLOV protein is shown as blue cartoon (PDB: 4EES). Additional LOV domain structures are shown in different shades of grey (from light to dark grey: LOV domain of YF1 (PDB: 4GCZ), Chlamydomonas rheinhardtii phototropin LOV1 (PDB: 1N9L), LOV2 domain of Avena sativa phototropin 2 (PDB: 2V0U). The residues corresponding to Y416, Y459, Y484 and W467 of parental iLOV are shown in stick representation in the corresponding color. The sequence logo shown in panel (B) was generated using the Skylign tool70 using a multiple sequence alignment containing 1975 LOV domain sequences taken from7. Sequence positions are numbered according to iLOV numbering. Amino acids relevant for the discussion are marked by blue (potential electron donating residues) and red (conserved glutamine Q489) asterisks and numbered in red.
