Figure 5
TIRF imaging of peptide labelling with FlAsH and AF647. (a) Immobilization scheme for anchoring FlAsH-FCMybbR and in-situ labelling with CoA-AF647. (b) TIRF image of FlAsH-FCMybbR immobilized on a coverslip coated with anti-His6x upon excitation at 473 nm (green spots). (c) TIRF image of CoA-AF647 when bound to FlAsH-FCMybbR after incubation with 1 μM Sfp and 10 mM MgCl2 upon illumination with 633 nm light (red spots). (d) TIRF image overlay displaying co-localization of the fluorescence signal of FlAsH and CoA-AF647 when both are bound to FCMybbR (yellow spots).
