Figure 7
FRET calculations based on molecular dynamics parameters. (a) MD-estimated centre-to-centre distance fluctuations between the FlAsH and AF647 labels bound to FCMybbR. (b) Computed orientation factor, κ2 calc, between the transition dipoles of FlAsH (blue arrow), and AF647 (red arrow) using equation (9). (c) Computed FRET efficiency over the span of 300 ns using the time-correspondent κ2 calc. from (b) and the distance from (a).
