Figure 9

ChIP and sequential ChIP. (A) Chromatin immuno-precipitation with anti-EZH2 followed by qPCR with NR2F2 (a) and OCT4 (b). The binding analysis shows that EZH2 binding increases on the NR2F2 promoter at D12 that later decrease at D20 while on the OCT4 promoter the EZH2 binding is maintained with differentiation (B) EZH2-OCT4 sequential ChIP followed by qPCR for genes EZH2 (a) and NR2F2 (b). The experiment confirms the c0-binding of EZH2 and OCT4 on the NR2F2 promoter at D12 that decreases slightly at D20. Values in both ChIP and sequential ChIP represent the average and standard deviation of two independent experiments and were calculated as percentage of total input. Error bars represent ± SEM, statistical significance represented as *(P < 0.5), **(P < 0.01), ***(P < 0.001).