Table 1 Primers designed and used in real-time reverse transcription-polymerase chain reaction (qRT-PCR) for amplifying polyamine-associated genes of barley plants.
Target gene | Fw Primer | Rv Primer | Reference |
|---|---|---|---|
HvHB1 (endogene) | TCGTCTTCAGCGAGGAGAAG | GATCTCGAAGATCTTGAGGAAG | |
Transgenic D-hordein:HvHB1 | GATCAATTCATTGACAGTCCAC | TCGCAGGTCATGACGAAGAC | |
ADC | CATCATCGTGTTGGAGATGG | AGCTTGTTGCTCTGGTCGAT | |
ODC | CGGCTCCAACTTCAATGG | GTCAGCTGGAGTAGGCCAAG | |
MAT | CTTCACCAAGCGTCCAGAA | GCATCAGCTCAGGGGTCTC | |
AdoMetDC | GGCTCTCTCATCTACCAGAGCTT | GATCTTGGCGACCCACTG | |
ACS1 | GTCTCCTCCCAGACGCAGTA | TGCGGGTGAAGTCCTTGT | |
ACS2 | GAGTTCAGACAGGCGATGG | GTCAAACCTGGCCTTCCAC | |
ACS5 | GAGCTGCTCACGTTCATCCT | CAAAACCCGGGTAGTACGG | |
ACS6 | TCCTCCAGCTCTACATCAAGC | GAGGAGGAGGCCGAAGTG | |
GADPH | TGTCCATGCCATGACTGCAA | CCAGTGCTGCTTGGAATGATG |
