Figure 6
From: IL-15 promotes human myogenesis and mitigates the detrimental effects of TNFα on myotube development
Antibody neutralisation of IL-15rα eliminates the myogenic effects of IL-15 and enhances the detrimental effects of TNFα on myotube development. Subconfluent myoblasts were switched to differentiation medium containing the indicated recombinant cytokines and antibodies (IL-15, 25 ng/mL; TNFα, 1 ng/mL; IL-15rα antibody, 1 µg/mL; IgG1 antibody, 1 µg/mL) for 8 d. Media were renewed every 2 d. Myotubes were fixed, immunofluorescence stained for desmin and DAPI and imaged on an epifluorescence microscope. (a) Myotube thickness data represents the mean ± SEM of 450 total measurements taken at 63x magnification from 90 myotubes per treatment condition (from 3 biological replicates). (b) Nuclear fusion index data represents the mean ± SEM values from 45 images taken at 20x magnification (from 3 biological replicates). *p < 0.05, ***p < 0.001 vs. IgG control condition by t-test.
