Figure 3
From: Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids
Construction of shMot-expressing adenovirus. (a) Schematic representation of the shMot-expressing adenoviral vectors. The RGD-incorporated adenovirus was generated by inserting RGD motif between HI-loop of the fiber knob (star). (ITR = inverted terminal repeat; Ψ = packaging signal; pA = polyA sequence; IX = protein IX; and shMot = mortalin-specific small hairpin (sh)RNAs). (b) Mortalin expression in keloid fibroblasts (KFs) after transduction with dE1-RGD/GFP/shMot. Scrambled shRNA not targeting any known human gene was used as a control. Overexpressed mortalin on the cytosolic and extracellular area of KFs, which were examined by immunofluorescence assay, were extremely knockdown by the treatment of dE1-RGD/GFP/shMot. Double immunostaining images of KFs show mortalin knockdown (mortalin, green; nucleus, blue; ×200). (c) The similar results were obtained on the primary keloid spheroids after infection of dE1-RGD/GFP/shMot (x680).
