Figure 5
From: Mortalin deficiency suppresses fibrosis and induces apoptosis in keloid spheroids
Knockdown of mortalin decreased PCNA expression and induced apoptosis. (a) Proliferating cell nuclear antigen (PCNA) immunohistochemical staining on keloid tissues (n = 3). (b) Expression of PCNA protein markedly increased in central and transitional keloid region compared to adjacent normal tissue by 3.9 times and 3.2 times (**p < 0.01). (c–e) Reduced expression of PCNA (c & d) and a marked increase in apoptosis as observed by TUNEL staining on the dEl-RGD/GFP/shMot treated keloid spheroids (c & e), and expressions of cytochrome C protein was increased by 4.4- and 1.8-fold after dEl-RGD/GFP/shMot transduction in comparison to untreated or scramble virus treated spheroid (c & f) (*p < 0.05; **p < 0.01). Original magnification: x400.
