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Figure 1

From: Morphology engineering of Streptomyces coelicolor M145 by sub-inhibitory concentrations of antibiotics

Figure 1

Morphology engineering by genetic approaches. (A) Confirmation of S. coelicolor M145 mutant for cslA by PCR using primers wh008-F and wh008-R. (M) marker, (1) 5897 bp amplified from wild type strain, (2) 4110 bp amplified from mutant strain. (B) Morphology of (a) wild type strain M145, (b) M145/pSET152, (c) M145/pSET152-cslA, (d) ΔcslA, (e) ΔcslA/pSET152, (f) ΔcslA/pSET152-cslA after five days of cultivation in TSB liquid medium. (C) Particles size of M145, M145/pSET152, M145/pSET152-cslA, ΔcslA, ΔcslA/pSET152, and ΔcslA/pSET152-cslA. (D) Act and Red production. Incubation was carried out in TSB liquid medium at 30 °C for five days. Data represented the average and standard deviation of three independent experiments.

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