Table 1 Primers used for qPCR analysis of mRNA and genetic engineering.
Primer | Primer Sequence(5′-3′) |
|---|---|
hrdB-F | tctctgtcatggcgctcattg |
hrdB-R | ttccactgagtggccggaatc |
SCO2836-F | gccaagtggaaccggaagaag |
SCO2836-R | acggaggcgaagaagtcgtag |
cslA-up-arms-F | cgacggccagtgccaagctttctccgggttcgttgacgatg |
clsA-up-arms-R | accaagggcgatctcatatgtcgtacttcgggagcgtcttc |
cslA-down-arm-F | catatgagatcgcccttggtggacttg |
cslA-down-arms-R | tcgacctgcagcccaagcttgactgcaccaggttcttaccc |
wh008-F | tggtggcctcgttgtggatg |
wh008-R | acgcctacctctgggtcaag |
wh009-F | tagagtcgacctgcagcccaag |
wh009-R | gaattcgtaatcatgtcatagc |
wh010-F | gaccggtgcaggcgaaggaag |
wh010-R | tatgacatgattacgaattctcattccttacgtcccccaag |
wh011-F | tgggctgcaggtcgactctagatcgcaggtgcacgcggtcg |
wh011-R | cttccttcgcctgcaccggtcggatcctaccaaccggcacg |
wh012-F | cgacggccagtgccaagcttgagccgagtcgttcggaattg |
wh012-R | tcctcggtgaaccgagatctgaggacaactgggcgatgatg |
wh013-F | agatctcggttcaccgaggaattcatc |
wh013-R | tcgacctgcagcccaagcttgccctggtagtacgtgttgtc |
wh015-F | gctctccactgtcacggaaac |
wh015-R | caggtagaagagcgtgtaccg |
