Figure 4 | Scientific Reports

Figure 4

From: Identification of PTPN1 as a novel negative regulator of the JNK MAPK pathway using a synthetic screening for pathway-specific phosphatases

Figure 4

PTPN1 regulates JNK-related cell death response. (A) Flag-PTPN1 or Flag-PTPN1 D181A was expressed in 293 T cells. Control cells were transfected with an empty vector. Upon TNFα-stimulation, protein expression and JNK phosphorylation were each examined by immunoblotting. (B) JNK activity was assessed using a luciferase reporter gene assay. The data are presented as the mean ± SD (repeats were performed in triplicate). (**p < 0.005) (C) Flag-PTPN1 or FLANG-PTPN1 D181A was expressed in p65-deficient MEF cells. After TNFα-stimulation (7 h), dead cells were visualized with PI staining (7 µg/ml) and observed via microscopy. (D) The relative cell death ratio was plotted ( ± SD, n = 3). (*p < 0.05).

Back to article page