Figure 3 | Scientific Reports

Figure 3

From: Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis

Figure 3

Increased induction of arginase-1 in the lungs of bleomycin-exposed groups during IL-6 or OSM overexpression. Following intratracheal intubation of AdDL70, AdOSM and AdIL-6, alone, or in combination with bleomycin (0.03 U/mouse), mice were sacrificed after 7 and 21 days. (A,B) Immunoblot images and densitometry analyses of STAT3, pSTAT3 and arginase-1 of lung homogenates following 7 days of exposure. (C–E) Representative arginase-1 immunostaining and quantification as well as arginase-1 mRNA signal in lung tissues following 7 days of exposure. Bar graphs represent mean ± SEM from 3–6 mice per group. All samples were derived at the same time and processed in parallel. The appropriate Western blot area depicting the antibody band was cropped and enclosed by black boxes, as indicated above. *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.05; ##P < 0.01; ###P < 0.001; *represent a difference between bleomycin-exposed groups and their respective controls (AdDL70- Bleo vs. AdDL70, AdOSM-Bleo vs. AdOSM, AdIL-6-Bleo vs. AdIL-6); #Represent a difference between the indicated groups. Significance was established using GraphPad, Prism 7.0 with one-way ANOVA using Newman-Keuls Multiple Comparison test.

Back to article page