Figure 6 | Scientific Reports

Figure 6

From: Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis

Figure 6

IL-6 increases the expression of pro-fibrotic factors in IL-4/IL-13 stimulated BMDMs. BMDMs were cultured from naïve C57BL/6 for 30 hours with recombinant IL-6 plus IL-4/IL-13. The harvested RNA and cellular supernatant were later examined for gene expression and cytokine/chemokine mediators, respectively. Anti-fibronectin antibody was used to stained lung tissues (Day 7). Significantly regulated pro-fibrotic related genes include (A) Fn1 (B) Mrc1 (C) Timp-1 and (D) Mcp-1. Analysis of mediators showed (E) MCP-1 protein level to be differentially regulated. (F) Representative images showing fibronectin positive staining. Bar graphs represent mean ± SEM from 3–5 samples per group, *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.05; ##P < 0.01; ###P < 0.001; *represent a difference between any sample relative to the control (control vs. IL-4/IL-13 or IL-4/IL-13/IL-6); #represent a difference between the indicated groups. Significance was established using GraphPad, Prism 7.0 with One-way ANOVA using Newman-Keuls Multiple Comparison test.

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