Table 1 Purification of AMCase from pig stomach.

From: Protease resistance of porcine acidic mammalian chitinase under gastrointestinal conditions implies that chitin-containing organisms can be sustainable dietary resources

Purification step

Total activity (mU)

Total Protein (mg)

Specific activity (mU/mg)

Yield (%)

Total soluble fraction

542.9 ± 3.3

20.2 ± 1.5

26.8 ± 0.2

100

Chitin beads Flow through

61.5 ± 2.4

12.2 ± 2.1

5.0 ± 0.2

11.3

Purified enzyme

49.6 ± 2.1

0.023 ± 0.006

2174.0 ± 90.4

10.3

  1. The purified protein was prepared from 1 G of stomach tissue as described in the Methods.
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