Figure 3
From: Bortezomib promotes KHSV and EBV lytic cycle by activating JNK and autophagy
Bortezomib-induced autophagy promotes KSHV and EBV lytic cycle. (A) BC3 and Raji cells were treated with bortezomib in the presence or in the absence of 3-Methyladenine (3-MA) (5 mM) or chloroquine33 (10 μM) and viral lytic antigen expression (K-bZIP and ZEBRA) was assessed after 24 hours of treatment by western blot analysis. (B) BC3 and Raji cells were silenced for ATG5 (siRNAATG5) or scramble-treated (siRNASC) for 48 hours and K-bZIP and ZEBRA expression was analyzed following 24 hours of bortezomib treatment. β-Actin was used as loading control. The histograms represent the mean plus standard deviation (SD) of the densitometric analysis of the ratio of specific proteins on β-Actin of three different experiments.
