Figure 2 | Scientific Reports

Figure 2

From: Gene delivery of hypoxia-inducible VEGF targeting collagen effectively improves cardiac function after myocardial infarction

Figure 2

Controlled expressions of hVEGF-Flag-T2A-mCherry and CBD-hVEGF-Flag-T2A-mCherry fusion proteins under hypoxic conditions. HEK293T cells were transfected with various pLOXCMV-EP-based lentiviral vectors and incubated under normoxic or hypoxic conditions for 24 h. (A–C) The expression of mCherry and EGFP was examined by fluorescence microscopy. (A) A representative microscopic image for each condition is shown (scale bar = 100 μm). (B) Percentage ratio of mCherry positive cells to EGFP positive cells was examined as described in SI Materials and Methods. ***p < 0.001 versus CMV promoter. (C) The quantification of mCherry fluorescence intensity was performed as described in SI Materials and Methods. ***p < 0.001 versus CMV promoter. (D) VEGF concentration in the culture supernatants were analyzed by a human VEGF ELISA Kit. ***p < 0.001 versus CMV promoter.

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