Figure 3
Biological activity and binding ability of CBDhVEGF to collagen. (A) HUVECs were incubated with hVEGF or CBDhVEGF (0–2,000 pg/mL, n = 4 for each dose) for 48 h. The cell growth-promoting activity was examined by CC-K8 Cell Counting Kit and expressed as the percentage of cell viability of resting cells. Results are presented as mean ± SEM of three separate experiments conducted in duplicate, **p < 0.01 versus control group. (B) The amount of hVEGF or CBDhVEGF bound to collagen was measured by ELISA assay (n = 4 for each dose). Results are presented as mean ± SEM of three separate experiments conducted in duplicate, **p < 0.01 versus hVEGF. (C) The K d values for binding of hVEGF or CBDhVEGF to collagen were calculated by Scatchard analysis. Slope of each line = −1/K d. (D Collagen-coated plates were washed extensively after incubation with hVEGF or CBDhVEGF. The effects of retained hVEGF or CBDhVEGF in collagen on HUVECs growth were determined by cell counting assay (n = 4 for each dose). Results are presented as mean ± SEM of three separate experiments conducted in duplicate, *p < 0.05, **p < 0.01 versus hVEGF.
