Figure 6 | Scientific Reports

Figure 6

From: Gene delivery of hypoxia-inducible VEGF targeting collagen effectively improves cardiac function after myocardial infarction

Figure 6

Expression of hVEGF in hypoxic myocardium and serum. (A) The expression of hVEGF in hypoxic myocardium was examined by immunohistochemistry using anti-human VEGF-A antibody. Representative images show increasing expression of hVEGF in mice treated with lentivirus expressing hVEGF, especially CBDhVEGF, compared with mice treated with control virus (scale bar = 50 μm). (B) Human VEGF production in serum was examined by a human VEGF ELISA assay. Results are presented as mean ± SEM (n = 8), ***p < 0.001. (C-E) Co-localization immunofluorescent staining of collagen I (red) and Flag (green) in the infarcted myocardium (C), liver (D) and descending thoracic aorta (E) of mice with injection of pLOX5HRE-hVEGF or pLOX5HRE-CBDhVEGF lentivirus for 2 to 6 weeks.

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