Figure 3 | Scientific Reports

Figure 3

From: Extensive gain and loss of photosystem I subunits in chromerid algae, photosynthetic relatives of apicomplexans

Figure 3

2D-PAGE of membrane complexes from Chromera solubilised by different detergents. Gel strips from CN-PAGE (see Fig. 2) were further separated in a second dimension by SDS-PAGE and stained with Coomassie blue; only the upper part of the gel is shown for the β-DM and β-DM/SDS samples. Individual spots were identified by MS. The PSI-PSII supercomplex (SC) observed after solubilisation with mild α-DM detergent was almost completely disrupted by β-DM solubilisation. Further addition of SDS resulted in the release of two antenna proteins from PSI (marked by red asterisks), however two superoxide dismutases (SOD1 and SOD2) and three other putative PSI subunits (Un1-3) remained bound to PSI core. All identified protein spots are listed in Table 1.

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