Figure 4

Detection of FeSOD activity in Chromera membrane complexes separated by CN-PAGE. Membrane were solubilised by β-DM and for each line 15 μg of chlorophyll was loaded. Membranes (3 μg of chlorophyll) isolated from the cyanobacterium Synechocystis 6803 were used as a negative control. After separation, the gel was stained for SOD activity essentially as described in ref.96. FeSOD enzymes are inhibited by peroxide but not by KCN, which however inhibits MnSOD enzymes. ZnSODs are inhibited neither by peroxide or KCN96.