Figure 5
From: RNA editing by ADAR1 regulates innate and antiviral immune functions in primary macrophages
ADAR1 knockdown macrophages present a pro-inflammatory phenotype that inhibits HIV-1 replication of bystander cells. (a) Cytokine expression profile of ADAR1 knockdown macorphages. Relative mRNA expression of the different cytokines normalized to siNT-transfected macrophages is depicted. Data represents mean ± SD of 3 different donors. *p < 0.05. (b) Relative mRNA expression of cytokines identified as upregulated in ADAR1 knockdown macrophages (IFNG, IL12A, IL1A and IL6). Data represents mean ± SD of 6 independent donors and is normalized to mock-transfected macrophages. ns; non-significant, **p < 0.005. (c) Antiviral activity of culture supernatants from ADAR1 knockdown macrophages. Percentage infection of untreated macrophages incubated with different concentrations of culture supernatants from mock- (white bars), siNT- (grey bars) and siADAR1- (black bars) treated macrophages. SN; supernatant, *p < 0.05.
