Figure 1 | Scientific Reports

Figure 1

From: Quantitative microglia analyses reveal diverse morphologic responses in the rat cortex after diffuse brain injury

Figure 1

Iba1 stained microglia in cortex regions after midline experimental diffuse brain injury and days of recovery. (a) Schematic drawing depicting the midline fluid percussion injury (mFPI) delivered to animals and the brain regions (impact, somatosensory barrel field [S1BF], and remote) imaged within cortical layers I-III. The arrows illustrate the percussive force delivered by the mFPI model. (b) Approximate imaging regions per histological section. (c) Iba1 immunostaining of impact, S1BF, and remote brain regions in sham animals and 1, 7, and 28 days post-injury (DPI). Cropped photomicrographs corresponding to the highlighted square are provided to show microglia morphology detail. All quantitative skeleton analysis was carried out on full sized photomicrographs while fractal analysis was carried out on single cells. (Scale bars = 10 µm unless otherwise noted).

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