Figure 3 | Scientific Reports

Figure 3

From: Quantitative microglia analyses reveal diverse morphologic responses in the rat cortex after diffuse brain injury

Figure 3

FracLac for ImageJ to quantify cell complexity and shape. (a) Illustration of FracLac box counting method to derive fractal dimension calculations of a microglia outline. Shape detail is quantified as scale increases, represented by pink boxes. Box counting equation is summarized in Table 1. (b) Schematic of the convex hull (blue), bounding circle (pink) and convex hull ellipse (orange) with accompanying longest length and width (dashed lines) necessary for calculating microglia span ratio and density (Table 1).

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