Figure 4
Microglia ramification is different in brain regions following experimental diffuse brain injury. (a) Summary data (mean and SEM) and statistical analysis (two-way ANOVA) of microglia cell counts/field in brain regions following diffuse brain injury (image n = 6/group, animal n = 3/group). At 1, 7, and 28 days post-injury (DPI), microglia counts are higher than sham within regions (F(2,24) = 9.07, p = 0.001) and with time (F(3,24) = 21.50, p < 0.0001) with significant interaction (F(6,24) = 5.08, p = 0.002). Post-hoc analyses are reported within the figure (# p = 0.003 vs impact and ^ p < 0.003 vs. remote). (b) Summary data and statistical analysis of microglia endpoints/cell at 1, 7, and 28 DPI (n = 3/group). The number of microglia endpoints/cell is different than sham after mFPI (F(3,24) = 33.67, p < 0.0001). There are fewer endpoint/cell in the impact and S1BF regions at all DPI, but not at the remote region (post hoc are summarized in figure). The number of endpoints/cell was also different among brain regions at time points (F(2,24) = 6.56, p = 0.005) with post-hoc analysis reported in figure (1DPI ^ p < 0.05 vs S1BF and Impact; 7DPI # p < 0.05 vs Impact and S1BF). There was a significant interaction effect (F(6,24) 3.17, p = 0.02) c). Summary data and statistical analysis of microglia process length/cell in brain regions at 1, 7, and 28 DPI (n = 3/group). Summed process length is different than sham in the days following mFPI (F(3,24) 21.78, p < 0.0001). Process length is decreased at all DPI in the impact and S1BF regions, but not the remote region (post hoc analyses are summarized in the figure). Microglia process length/cell was also different among brain regions at time points (F(2,24) 9.99, p = 0.0007) with post hoc analysis reported in figure (1DPI ^ p < 0.05 vs S1BF and Impact; 7DPI # p < 0.05 vs Impact and S1BF; 28DPI ^ p = 0.04 vs Impact). There was a significant interaction effect (F(6,24) = 3.07, p = 0.02).
