Figure 5
Microglia complexity, elongation, and size are different in brain regions following experimental diffuse brain injury. (a) Example photomicrographs and cell outlines of Iba1/DAB microglia in impact, S1BF, and remote regions; scale bar = 10 µm. (b) Summary data and statistical analysis of fractal dimension at 1, 7, and 28 DPI. Microglia fractal dimension was different than sham post-mFPI within brain regions (F(3,24) = 2.99, p = 0.05), decreased in the S1BF region after 1 DPI and 7 DPI, and increased in the remote region at 7 and 28 DPI. Microglia fractal dimension was also different according to time (F(2,24) = 84.07, p < 0.0001). There was a significant interaction effect (F(6,24) = 5.53, p = 0.0001). All post-hoc analyses are reported in the figure (Sham: # p < 0.01; 1DPI: * p < 0.05 vs impact and S1BF; 7DPI: ^ p < 0.05 vs Impact and S1BF; 28DPI: % p < 0.05 vs Impact and S1BF). c) Summary data and statistical analysis of span ratio at 1, 7, and 28 DPI. Microglia span ratio was different than sham within brain regions in the days following mFPI (F(3,24) = 10.43, p < 0.0001), elongated in the S1BF region after 1, 7 and 28 DPI. Microglia span ratio was also different between brain regions (F(2,24) = 43.15, p < 0.0001). All post hoc analyses are reported in the figure (1DPI: # p < 0.05 vs S1BF; 7DPI: * p < 0.0001 vs S1BF; 28 DPI: ^ p = 0.05 vs S1BF). There was a significant interaction effect after mFPI (F(6,24) = 8.9, p < 0.0001). (d) Summary data and statistical analysis of density at 1, 7, and 28 DPI. Microglia density was different than sham within brain regions after mFPI (F(3,24) = 5.28, p = 0.006). Microglia density was decreased when in the S1BF region at 1 DPI when compared to sham but increased at 28 DPI. Microglia density was also different between brain regions (F(2,24) = 46.54, p < 0.0001). There was a significant interaction effect after mFPI (F(6,24) = 6.11, p = 0.0005). All post hoc analysis reported in figure (Sham: # p < 0.01; 1DPI: * p < 0.05 vs S1BF and Remote; 7DPI: ^ p < 0.01 vs S1BF and Remote; 28DPI: % p < 0.05 vs S1BF and Remote). All data are mean ± SEM with two-way ANOVA analysis.
