Figure 9

Effect of exogenous hematin on growth, Na⁺ concentration and SOS1 expression in fc1 mutants under salt stress. (A,B) Wild-type and fc1 mutant plants after germination were grown in MS medium containing 0 or 2 μM hematin with or without 100 mM NaCl for 14 d. Exogenous hematin effect was assayed as the whole plants fresh weight when treated with or without hematin. C: Germination rate of WT and fc1 seeds with 100 mM NaCl and/or 2 μM hematin for 7 d. D: Fresh weight of WT and fc1 seeds with 100 mM NaCl and/or 2 μM hematin for 7 d. E: Three-week-old wild type and fc1 mutant plants were treated with 100 mM NaCl with and/or 2 μM hematin for 3 days. Then sodium ion contents in whole plants were detected. F: Two week-old WT and fc1 mutant plants were treated with 0 and 200 mM NaCl with and/or 2 μM hematin for 4 h. Total RNA was isolated from the plants and analyzed by qRT-PCR. SOS1 transcript level was analyzed by qRT-PCR. Vertical bars represent Values represent mean values ± SE. Asterisk indicates the significant difference between fc1 mutant and wild type (p < 0.05).