Figure 1
From: Modeling and Preventing Progressive Hearing Loss in Usher Syndrome III
Generation of KO-TgAC1 mice. (A) A schematic of a transgene construct. The transgene construct is composed of an Atoh1 enhancer sequence fused to the beta globin basal (GB) promoter sequence. The Clrn1 cDNA fused downstream of the Atoh1 regulatory elements is composed of the 5′ UTR sequence, the coding sequence (isoform 2) and the 3′ UTR sequence. (B) The breeding scheme used to generate KO-TgAC1 mice. The expanded symbol for the KO-TgAC1 mice is “TgAC1; Clrn1 KO/KO”, marked by an asterisk in the F2 generation. The number next to the mouse corresponds to the lane number in panel C. (C) PCR-based genotyping to identify the WT (2066 bp), KO (782 bp) and TgAC1 (1938 bp) allele of Clrn1. *F2 mice with the desired genotype, TgAC1; Clrn1 KO/KO.
