Figure 5

MKL1-Tg mice developed fulminant DSS-induced colitis. MKL1-Tg mice and non-Tg littermates (8 weeks old) received 3% DSS in their drinking water for 7 days. (A) Male body weights were measured on day 0, 1, 4, and 7, and are shown as the percentage of initial body weight. (B) Representative image of whole colons from female MKL1-Tg mice and control littermates. Colon length was measured (n = 3 for each group). (C) Representative histological images (low-power field) of Swiss-rolled whole colon stained with H&E. Regions specified with blue lines were affected by colitis. Positions of anuses are indicated by circles. Lesion area was measured and plotted (n = 5 for each group). (D) Representative histological images (high-power field) of the proximal region of the colon stained with H&E. We calculated the histological score (n = 5 for each group). (E) MKL1-Tg mice and non-Tg littermates received 3% DSS in the drinking water for 4 days. Representative cytograms showing Ly6C and MHC-II expression on the CD45⁺CD11b⁺CD11c^{low to mid} CD64⁺ cells. We calculated the proportions of LPMac. Experiments were repeated 3 times. Data are shown as mean ± SD. *P < 0.05.