Figure 4 | Scientific Reports

Figure 4

From: Novel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins

Figure 4

SDS-PAGE analysis of clMagR-lipase, clMagR-α-AF, clMagR-pullulanase. (a) lane M: standard marker proteins; lane 1: supernatant of cell lysate (BL21-pET28a); lane 2: precipitation of cell lysate (BL21-pET28a); lane 3: supernatant of cell lysate (BL21-p28aMagR-lipase); lane 4: precipitation of cell lysate (BL21-p28aMagR-lipase); lane 5: the purified clMagR-lipase protein (the single band marked with the arrow); (b) lane M: standard marker proteins; lane 1: supernatant of cell lysate (BL21-pET28a); lane 2: precipitation of cell lysate (BL21-pET28a); lane 3: supernatant of cell lysate (BL21-p28aMagR-α-AF); lane 4: precipitation of cell lysate (BL21-p28aMagR-α-AF); lane 5: the purified clMagR-α-AF protein (the single band marked with the arrow); (c) lane M: standard marker proteins; lane 1: supernatant of cell lysate (BL21-pET28a); lane 2: precipitation of cell lysate (BL21-pET28a); lane 3: supernatant of cell lysate (BL21-p28aMagR-pullulanase); lane 4: precipitation of cell lysate (BL21-p28aMagR-pullulanase); lane 5: the purified clMagR-pullulanase protein (the single band marked with the arrow).

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