Table 2 Enzyme activity of lipase, α-AF and pullulanase. aclMagR-enzymes were purified by iron beads. bclMagR was removed from enzymes. cFree enzymes were purified by Ni-NTA affinity chromatography. dHis tag was removed from free enzymes.
Sample | Specific activity (U/mg) | Purification (fold) |
|---|---|---|
Crude extract | 187.2 ± 14.5 | 1.0 |
clMagR-lipasea | 1795.3 ± 172.8 | 9.6 |
Lipaseb | 1589.1 ± 141.7 | 8.5 |
Free lipasec | 1309.3 ± 112.6 | 7.0 |
Free lipased | 1487.5 ± 153.8 | 7.9 |
Crude extract | 15.0 ± 2.2 | 1.00 |
clMagR-α-AFa | 139.5 ± 17.4 | 9.3 |
α-AFb | 118.5 ± 14.3 | 7.9 |
Free α-AFc | 87.0 ± 9.7 | 5.8 |
Free α-AFd | 89.2 ± 8.5 | 5.9 |
Crude extract | 2.85 ± 0.57 | 1.00 |
clMagR-pullulanasea | 23.09 ± 3.85 | 8.10 |
Pullulanaseb | 18.47 ± 3.29 | 6.48 |
Free pullulanasec | 12.20 ± 2.44 | 4.28 |
Free pullulanased | 12.31 ± 2.23 | 4.32 |
