Figure 1
From: Glioblastoma cancer stem cell lines express functional acid sensing ion channels ASIC1a and ASIC3
ASIC1 and ASIC3 are expressed in GSC lines. (a) Left, RT-PCR analysis revealed expression of ACCN2a, ACCN2b and ACCN3 but not ACCN1 in R8 and R54 cells respectively. HPRT (hypoxanthine-guanine phosphoribosyltransferase) served as a reference gene. Right, qPCR analysis revealed different expression levels of ACCN2a, ACCN2b and ACCN3 in R8 and R54 cells. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) served as a reference gene. (b) Left, western blot analysis revealed expression of ASIC1 and ASIC3 in GSC lines, ß-actin was used as control. Right, summary of four Western blots for ASIC1 and three Western blots for ASIC3; expression was normalized to ß-actin.
