Figure 2
From: Glioblastoma cancer stem cell lines express functional acid sensing ion channels ASIC1a and ASIC3
Electrophysiological characterization of ASICs in R54 cells. (a) Left, whole cell current traces representative for type 1, type 2, and type 3 currents, activated by different acidic pH; conditioning pH was 7.4. Right, pH-response relationships. Peak currents from type 1 and type 2 cells were normalized to the current at pH 6.0. At pH ≥ 6.5, in most cases there was no transient current, and the amplitude of the inward current upon acidification was measured. The curves represent fits to the Hill equation. Amplitudes of sustained currents from type 2 and type 3 cells were determined just before stepping back to pH 7.4 and were connected by lines. (b) Pie chart illustrating the relative occurrence of type 1, type 2, and type 3 currents. (c) Frequency histogram of peak amplitude of type 1 (dark blue) and type 2 (light blue) transient currents. (d) Time constants of desensitization τdes of transient type 1 and type 2 currents.
