Figure 4
From: Glioblastoma cancer stem cell lines express functional acid sensing ion channels ASIC1a and ASIC3
Electrophysiological characterization of ASICs in R8 cells. (a) Left, whole cell current traces representative for type 2 and type 3 currents, activated by different acidic pH; conditioning pH was 7.4. Right, pH-response relationships. Peak currents from type 2 cells were normalized to the current at pH 6.5. The curves represent fits to the Hill equation. Amplitudes of sustained currents from type 2 and type 3 cells were normalized to pH 5 and were connected by lines. (b) Pie chart illustrating the relative occurrence of type 2 and type 3 currents. (c) Frequency histogram of peak amplitude of type 2 transient currents. (d) Time constants of desensitization Ï„des of transient type 2 currents.
