Figure 5
Comparative determination of internalization capacity of anti-PLAC1 antibody and its ADC. The degree of PLAC1 mAbs internalization in DU145, LNCaP, and PC3 cells was investigated by flow cytometric method at various time points. After each time point, the proportion of internalized fraction was measured (a). Comparative internalization of anti-PLAC1 antibody following binding to the surface PLAC1 is shown in LNCaP, DU145 and PC3 prostate cancer cells. LS180 was assessed in parallel as PLAC1 negative cell line (b). Internalization of anti-PLAC1 antibody and its SN38 conjugate after engagement with surface PLAC1 in LNCaP cells was tested and compared (c). Data were generated from three independent experiments. Internalization of anti-PLAC1 antibody in LNCaP cells was visualized at different time periods by immunofluorescent staining. A progressive accumulation of the fluorescent signal within cells indicates antibody internalization (d).
