Figure 1
Spine densities of wild-type and CaMKIIα KI hippocampal neurons maintained in slice cultures. (a) Representative images of oblique, apical, and basal dendrites at different developmental stages. Scale bars, 5 µm. (b–d) Quantitative analysis of spine density in hippocampal pyramidal neurons maintained in slice cultures of wild-type and CaMKIIα KI mice at 9/10 DIV, 16/17 DIV, 23/24 DIV and 30/31 DIV. Spine density was measured from images of oblique (b), apical (c), and basal (d) branches of dendrites, collected from 7–22 experiments. Data are presented as the mean ± SEM, n = 7–22 cells, *p < 0.05, **p < 0.01 or ***p < 0.001, two-way ANOVA followed by Tukey’s post-hoc test. (e) The protein expression of CaMKIIα at 10, 13, 16 and 22 DIV in slices from KI and wild-type mice. CaMKIIα protein expression was low at 10 DIV in both KI and wild-type mice. A large increase in protein expression was observed from 10 to 16 DIV. Full-size images of the gels and blots are shown in Supplementary Fig. 1.
