Figure 6
Binding of MAMB1, MAMA2 aptamers and MGB2, MGB1 antibodies to nontransfected HEK293, MCF7, and MDA-MB-415 cells. (A) Mean of fluorescence intensity of non-transfected HEK293 cells (black), MCF7 (red), and MDA-MB-415 (blue) without probes (cells only). (B) Mean of fluorescence intensity of non-transfected HEK293 cells binding to MAMB1 (black), MAMA2 (green), MCF7 binding to MAMB1 (red), and MDA-MB-415 binding to MAMA2 (blue). (C) Mean of fluorescence intensity of non-transfected HEK293 cells binding to anti-MGB1 antibody (black), anti MGB2 antibody (green), MCF7 binding to anti MGB2 antibody (red), and MDA-MB-415 binding to MGB1 antibody (blue). (D) Mean of fluorescence intensity of non-transfected HEK293 cells (black), MCF7 (red), and MDA-MB- 415 (blue) binding to a random sequence. Cells were incubated with their corresponding probes and 10000 events was counted by flow cytometry. Then data was analyzed using flow cytometry C6 sampler software.
