Figure 7
Competition binding assays of MAMB1, MAMA2 aptamers and anti MGB, anti MGB1 antibodies on transfected HEK293 cells with MGB2 and MGB1 plasmids. (A) Mean of fluorescence intensity of transfected HEK293 with MGB2 plasmid (Trans B) binding to anti MGB2 (orange) and MAMB1 (purple), and transfected HEK293 with MGB1 plasmid (Trans A) binding to anti MGB1 (green) and MAMA2 (brown). (B) Mean of fluorescence intensity of Trans B and Trans A HEK293 blocked with unlabeled MAMB1 and MAMA2 aptamers respectively, and probed with anti MGB2 (red) in the case of Trans B, and anti MGB1 (brown) in the case of Trans A. Mean of fluorescence intensity of Trans B and Trans A binding to MAMB1 (purple), and MAMA2 (brown) same as in A. (C) Mean of fluorescence intensity of Trans B and Trans A HEK293 blocked with random sequence (RAN) and probed with anti MGB2 (dark yellow) in the case of Trans B and anti MGB1 (light blue) in the case of Trans A. Mean of fluorescence intensity of Trans B and Trans A binding to MAMB1 (purple) and MAMA2 (brown) same as in A. Cells were incubated with their corresponding probes and 10000 events was counted by flow cytometry. Then data was analyzed using flow cytometry C6 sampler software.
