Figure 2

Double CD4/IL-17A and CD4/Foxp3 immunostainings. Panel 1: Confocal microscopy image of CD4⁺/IL-17A⁺ lymphocytes. (a) IL-17A⁺ lymphocytes, (b) CD4⁺ lymphocytes, (c) merged images (CD4 show a membrane immunostaining while IL-17A show a cytoplasmic one). The insertions show image amplification. (d) and (e) IL-17A and CD4 isotype control images, respectively. Panel 2: Epifluorescence light microscopy image CD4⁺/Foxp3⁺ lymphocytes. (a) CD4⁺ lymphocytes, (b) false red colour, (c) Foxp3⁺ lymphocytes, (d) DAPI counterstaining and (e) merged images (the insertion shows image amplification). (f), (g) and (h) isotype control images.