Figure 2 | Scientific Reports

Figure 2

From: Therapy of corneal endothelial dysfunction with corneal endothelial cell-like cells derived from skin-derived precursors

Figure 2

Derivation of CEC-like cells from SKPs. (A) During co-culturing, cell morphology began to change to a polygonal CEC-like shape on day 4. After 8 days, the polygonal CEC-like cells were the majority and formed a mosaic monolayer. (B) Immunofluorescent staining showed that the CEC-like cells expressed corneal endothelium major markers Na+/K+ ATPase and ZO-1. (C) RT-PCR analysis of corneal endothelial markers in CEC-like cells and SKPs. (D) Expression of Pitx2 and FoxC1 during derivation. (E,F) Western blotting analysis and protein levels of Na+/K+ ATPase alpha 1 and ZO-1 in CEC-like cells compared to SKPs. All samples derived from the same experiment were processed in parallel. One representative experiment out of three is presented. Cropped images were displayed and full-length blots are shown in the Supplementary Figure 4. Results are mean ± S.E.M. *p < 0.05 compared to SKPs.

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